ДП «Науковий центр превентивної токсикології, харчової та хімічної безпеки імені академіка Л.І. Медведя МОЗ України», м Київ, Україна

РЕЗЮМЕ. Мета. Обґрунтувати підходи з'ясування проблеми оцінки негативного впливу відходів на здоров'я населення та навколишнє середовище, який виникає з моменту утворення і на всіх етапах поводження з відходами.

Матеріали та методи. Одним з пріоритетних напрямків наближення до вимог Європейського Союзу при поводженні з відходами є гармонізація сучасного українського законодавства до європейських стандартів, адаптація положень нормативно-правових актів, у тому числі класифікація відходів за ступенем небезпеки. У статті проводиться порівняльний аналіз законодавчої бази з оцінки небезпеки відходів існуючої в даний час в Україні з рекомендаціями ВООЗ та Директивами ЄС. Обґрунтовані і рекомендовані для впровадження методичні принципи віднесення відходів до певної класифікаційної категорії за небезпекою.

Результати та висновки. Науково обґрунтовано, що віднесення відходів до певної класифікаційної категорії за небезпекою повинно ґрунтуватися не тільки на кількісних розрахунках за хімічним складом і токсичності складових інгредієнтів, які входять до складу відходів, а й на експериментальній перевірці зразків з використанням альтернативних методів.

Ключові слова: відходи, Директиви ЄС, класифікація, біотестування, токсичність, небезпека, альтернативні методи.

Introduction. During the last 10–15 years there has been a steady trend in adverse effects of various environmental factors on human health, leading to negative effects — health deterioration, increased morbidity, and, sometimes, mortality. There is no doubt that the contaminated environment is the media which impairs the health and well-being of people who are weakened or have chronic diseases. Today we will talk about human waste, industrial waste, agricultural technology waste and others — they are among the most dangerous pollutants. In the media, from the tribunes of scientific and environmental forums the words “Curse of our time” — that's what the waste (the consequences of human management) is called, are heard more and more often [1, 2].

The Aim of the Research. Clarification of the problem of the negative impact of waste on the environment and public health. They appear from the waste formation and at all stages of its handling.

Materials and Methods. One of the priority areas of approximation to the requirements of the European Union in waste management is the harmonization of modern Ukrainian legislation with European standards, adaptation of regulations, including the classification of waste according to the degree of danger. The article provides a comparative analysis of the legal framework for waste hazardousness assessment currently existing in Ukraine with WHO recommendations and EU Directives. The methodological principles of classification of waste according to a degree of hazardousness are substantiated and recommended for implementation.

Results. According to the State Statistics Service in Ukraine today:

• There is about 300 tons of waste per each inhabitant, and this amount is constantly increasing;

• 13 billion tons of waste have been accumulated;

• There are, by far incomplete data, about 35 000 illegal dumps.

These are eloquent figures, so the whole world has started to look for a solution for this problem, the urgency of which is out of the question. It is obvious.

In the EU the waste management law is presented in ten directives and other regulations [3]:

• Directive 2008/98/EC on waste (Waste Framework Directive)
• Directive 1999/31/EC on the landfile of waste
• Directive 2006/21/EC on the management of waste from extractive industries
• Directive 96/59/EC on the disposal of polychlorinated biphenyls and polychlorinated terphenyls (PCB/PCT)
• Directive 94/67/EC on the incineration of hazardous waste
• Directive 2012/19/EC on waste electrical and electronic equipment
• Directive 2006/66/EC on batteries and accumulators and waste batteries and accumulators
• Stockholm Convention on POPs (ratified in Ukraine on 18.04.2007 № 949-V)
• Basel Convention on the Control of Transboundary Movements of Hazardous Wastes and their Disposal (Ukraine's Law
• Directive 94/62/EC on packaging and packaging waste
• Directive 91/689/EC on hazardous waste №803-XIV from 07.01.99).
• Rotterdam Convention on the Prior Informed Consent Procedure for Certain Hazardous Chemicals and Pesticides in International Trade (entered into force in Ukraine on December, 1 2019).

In September 2014 the directive of the Cabinet of Ministers of Ukraine “On the implementation of the EU-Ukraine association agreement” approved a road map for the implementation of EU Directives, developed and submitted to the Cabinet of Ministers of Ukraine drafts of regulations for the implementation of the provisions of Directives that are specified in the Annex to the Agreement [4].

Ukraine, first of all, should implement the following Directives:

• Directive 2008/98/EC of the European Parliament and the European Council “On waste and repealing certain Directives” (Framework) of 19 November 2008, which provides for tightening of requirements for waste collection and recycling. Based on the proposals of the European Commission, the following requirements were set: re-use and recycling of 65% of total waste and 10% of permissible waste disposal in the European Union;
• Directive 1999/31/EC on the landfill of waste — waste management policy provides that in the future within the Community only safe and controlled activities on waste disposal should be performed; whereas, in accordance with the “polluter pays” principle, any damage to the environment resulting from the disposal of waste must be taken into account;
• Directive 2006/21/EC on the management of waste from extractive industries sets out recommendations for the prevention and minimization of risks that may arise from the generation and disposal of extractive waste.

The terms of implementation of these directives range from 2 to 5–6 years.

The EU Directives set out the basic principles for the management of waste and its individual fractions. The base point for all directions is the classification of waste according to hazard degree that is necessary to:

• conduct proper listing and certification of waste
• determine the fee for their placement and storage
• provide rationale for the design and determination of landfill locations
• assess the degree of danger to the environment
• assess the danger to human health in the cause of waste handling
• develop differentiated approach in making decisions for reasonable and secure way of waste management — storage, recycling, disposal.

Problems of negative impact of waste on the environment and public health in the places of its formation and accumulation may occur from the moment of formation, at all stages of its handling and up to the moment of disposal or recycling.

In this case, the following risks should be taken into account:

• at the stage of formation
• at the stage of storage (violation of conditions and terms)
• at the stage of transportation (transportation conditions — contact with oxygen, oxidation processes, migration of monomers from polymers)
• at the stage of recycling — use of technologies that do not meet European standards, which can lead to environmental pollution
• obtaining raw materials and products from waste (toxicity indicators)
• the risk of occupational diseases in the process of dealing with waste.

According to the EU Hazardous Waste Directive, “waste is classified as hazardous if it contains certain hazardous chemicals or has properties that can make it hazardous”.

When assigning waste to certain classification risk categories according to EU Directives, origin and constituent ingredients, that can make them dangerous, should be taken into account.

According to the recommendations of the UN there are the following hazard categories of chemicals [5]:

• explosive substances and products
• flammable substances
• flammable solids
• substances prone to spontaneous ignition
• substances that emit flammable gases in contact with water
• oxidizing substances
• organic peroxides
• toxic substances
• infectious substances
• radioactive materials
• corrosive substances
• other dangerous substances and products.

Current European legislation in accordance with Article 2 of the European Commission Decision 2000/532/EC recommends that when assessing the hazard of waste the percentage of one or more of the mentioned components be taken into account, if they are classified as [6, 7]:

• potent toxic substances > 0.1%;
• toxic substances > 3.0%;
• hazardous substances > 25.0%;
• caustic substances (symbol — R35) > 1.0%;
• caustic substances (symbol — R34) > 5.0%;
• irritants (symbol — R41) > 10.0%;
• irritants (symbols — R36, R37, R38) > 20.0%;
• class 1 or 2 carcinogen > 0.1%;
• class 3 carcinogen > 1.0%;
• substances that exhibit class 1 or 2 reproductive toxicity (symbols — R60, R61) > 0.5%;
• substances that exhibit class 3 reproductive toxicity (symbols — R62, R63) > 5.0%;
• class 3 mutagen (symbol — R40) > 1.0%;
• class 1 or 2 mutagen (symbol — R46) > 0.1%.

SGS SA (Société Générale de Surveillance) is a Swiss company that provides independent examination, control, testing and certification services (the total number of staff of the Ukrainian division of SGS is 1,200 people) and allows to classify substances by such hazard factors based on known data on dangerous properties of chemicals and mixtures. SGS SA was created to bring the hazard criteria of substances, used in different countries, to a single standard. In complex cases, reliable epidemiological data and information on the effects of substances on humans are taken into account, and in the case of mixtures for which reliable test data may be missing, the method of interpolation of the properties of the components of these mixtures is used. In terms of impact on the human body 9 hazard types as listed above are also defined (according to acute toxicity in a single oral, inhalation or transdermal application).

Based on the analysis of the presence or absence of the above-mentioned toxicity criteria, certain hazard classification category is assigned to the components of the waste. According to the appendix 3 of the Directive on hazardous waste when assessing the danger of waste, ecotoxicity should be among other properties, taken into account. There are two types of hazards for the environment — for the aquatic environment and the ozone layer.

Determination of the environmental hazard of waste allows taking into account both the direct danger of working with it and long-term risks when using different technologies for waste management, disposal or recycling. The presence of hazardous chemicals in human habitats is determined by the state of certain microorganisms most sensitive to each type of pollutants. The toxicity of water, precipitation, wastewater, water extracts from soils is established by the change of the level of fluorescence of chlorophyll, algae cells. When enzyme systems were used as a test object (dehydrogenase inhibition assessment), rather high sensitivity of test reactions to the presence in water of high concentrations of heavy metals (mercury, lead, copper, cadmium) and some organic compounds (phenols, resorcinol, hydroquinone, etc.) was found.

Tetrahymena pyriformis W., as an in vitro test system, is widely used in toxicology as an alternative test object for detecting the toxicity of many environmental pollutants. As a rule, studies on infusoria are performed according to the criterion of “cell death” to determine the parameters of acute and chronic toxicity. In addition to the criterion of “cell death”, an important characteristic of the functional state of infusoria is their motor activity [8–12].

The list of some biotests which are conducted with conservation purposes is presented in Table. 1.

Table 1

The list of some biotests which are conducted with conservation purposes

At the First Congress of Russian Toxicologists a special workshop was devoted to the reports on alternative methods of study [13]. The use of alternative models in toxicology is recommended in three areas:

• identification of the toxicometry parameters for individual chemicals or mixtures of products in order to assess their toxicity;
• assessment of the degree of toxicity of water, bottom sediments, soils at multifactorial pollution;
• sanitary and toxicological assessment of industrial products, as well as industrial waste, as a stage of their sanitary-epidemiological examination.

The introduction of alternative models and methods in toxicology is supported by WHO and the International biomedical community. It is controlled by international organizations such as the European Centre for the Validation of Alternative Methods (ECVAM), the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the European Society of Toxicology In Vitro (ISTIV).

In many sectors of industry, when assessing the toxicity of waste, the amount of animal testing is gradually declining. The reliability of biotesting methods and their compliance with the tasks is coordinated by independent expertise in the fields of biology, chemistry, agrochemistry and pharmaceutics.

The advantages of biotesting methods are:

• universality, expressivity, simplicity, availability, low cost and high sensitivity;
• efficiency in receiving the answer;
• availability and representativeness of test objects.

The main test objects for the assessment of ecotoxicity are presented in Table 2.

For research purposes, according to the US Environmental Protection Agency (EPA), 4,650 tests with 145 test objects are performed for hazard assessments of waste.

The following alternative models have currently been developed:

1. Invertebrates

2. Hydrobionts

3. Microorganisms

4. Plants

5. Cultures of human and animal cells

The list of alternative test objects for toxicity assessment is presented in Table 3.

Presented in the literature data on the assessment of toxicity of a number of wastes performed on laboratory animals, as well as with biotesting on Daphnia Magna Straus and Scenedesmus quadricauda methods (Table 4) indicate a significant sensitivity of biotesting methods compared to studies conducted on laboratory animals [14].

Table 2

The main test-objects for the assessment of ecotoxicity

Note: * OECD 210 “Fish, Early-life Stage Toxicity Test”

** OECD 202 “Daphnia sp, Acute Immobilization Test”, OECD 211 “Daphnia magna Reproduction Test”

*** OECD 201 “Freshwater Alga and Cyannobacteria, Growth Inhibition Test”

Table 3

Alternative test objects for waste toxicity assessment

Designation of certain categories of waste hazard is a particularly acute issue for Ukraine, firstly, because of the lack of regulatory framework, and secondly — because of the mismatch between the EU classification of waste (hazardous, safe, inert) and the Ukrainian one (1–4 classes of danger). The existing so far in Ukraine method of determining waste hazard class for toxicological criteria (LD₅₀, the MPC in soil), physico-chemical parameters (volatility and solubility) does not take into account a number of factors of potential danger. Application of this technique for a full-range assessment of environmental risks was impossible. At present, the regulatory document, which determines hazard classes, is put on hold [15]. When assigning the degree of waste hazard the requirements set out in the Resolution of the Cabinet of Ministers №1120 on the Basel Convention are applied [16]. This document recommends assigning waste to yellow list (dangerous) or green list (safe) taking into consideration the constituent ingredients of waste. Lists of chemicals, developed by the International Organization for Economic Cooperation and Development (OECD) and the list of dangerous properties, are in line with European legislation. However, this classification is made and can be used only for the cross-border transportation of hazardous freight, as the data on the technology of waste production, ingredients and their consistency, storage conditions are often missing.

We have conducted comparative studies of the toxicity of water extracts on some animals — at a single intra gastric administration (at an average lethal concentration — LD₅₀) and on culture of unicellular Tetrahymena pyriformis W infusoria (death, %) (Table 5) [17, 18].

The results presented in table. 5, indicate a possible negative impact on the environment (percentage of infusoria death 5–100%) of some of the studied wastes (compost after the biodegradation of unfit and banned pesticides (UBP), premixes, black sludge, pyritic sulphur, drill cuttings), which allows to determine them as hazardous wastes. At the same time for these wastes no toxic effect was found in warm-blooded organisms at a single intragastric admission and transdermal application [19].

An analysis of the potential danger to the environment and human health of used car oil filters was conducted with the obtained results of their chemical and toxicological features studies. Mixed samples from used filters (oils and material of these filters) extracted from garbage containers at car service stations were used for the research. It is established that used car oils are of low-hazard for warm-blooded animals (hazard class 4 in accordance with GOST (State Standard) 12.1.007-76 at LD ₅₀ > 5000 mg/kg). Signs of irritative effect on skin were not found. However, using the method of biotesting, in the test for induction of chromosome aberrations in culture of human peripheral blood lymphocytes in vitro in experimental variants with and without metabolic activation a mutagenic activity of oil from used car filters was revealed [20, 21].

Table 4

Comparative evaluation of the results of research on the toxicity of waste in laboratory animals and with biotesting methods

Note: * DSanPin 2.2. 7.029-99 [15]

Table 5

The results of comparative studies of waste toxicity on Tetrahymena pyriformis W infusoria and laboratory animals

Note: * — GOST 12.1.007-76 (State standard) [19]

** — infusoria — % of death

Systematic dumping of this waste in landfills and dumps, when chemical processes take place under the influence of environmental factors (acidity, moisture, temperature, solar insolation) will facilitate the migration of toxic substances into ecosystems and cause negative effects for the environment and the population living in the surrounding areas. The threat of pollution and negative impact on groundwater and surface water is quite high. The above mentioned data allowed us to consider used car filters as toxic wastes, which requires, when working with them, stricter regulation in terms of collection and disposal, as well as control by the state.

The results of the research confirm the effectiveness of biotesting methods in assessing the hazardousness of waste in comparison to traditional chemical-analytical approaches in assessing the degree of pollution of ecosystems, which is consistent with European approaches to assessing the risks of pollutants to humans and their habitats.

Conclusions. 1. One of the priority areas of approximation to the requirements of the European Union on waste management is the harmonization of modern Ukrainian legislation with European standards, adaptation of regulations, in particular on production wastes and consumption residue.

2. An essential issue in the implementation of state waste management system in Ukraine is the development and implementation of directives on the methodology of assigning waste to a certain classification category by hazard, taking into account current WHO recommendations and European Directives on waste management.

3. Assignment of waste to certain classification categories for toxicity and danger for the environmental and public health should be based not only on quantitative calculations of the chemical composition of their hazardous chemical components, but also on biological experimental testing of samples.

4. The principles of wastes risk assessment methodology are:

• Integral assessment of total waste hazard resulting from contamination of all contacting environments;
• Comprehensive assessment of the waste as a single dangerous agent, inclusive of the dynamics and forms of its transformation in the environment;
• Consideration of the specific harmful effects of waste in each environment contacting with the human;
• Evaluation of waste constituent ingredients that defines the nature of their dangerous action.

5. Experimental determination of the degree of waste danger — a laboratory testing of the environmental toxicity of studied samples with the use of biological objects.

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Received 09/24/2020