Toxicological profile of propisochlor in a two-year experiment on Wistar Han male rats

  • Authors: O.V. Reshavska, N.M. Nedopytanska, Ye.A. Bahlii, T.V. Usenko, L.V. Tkachenko, T.I. Kliuchynska, T.V. Verbova
  • UDC: 615.9:616-006.6:632.95.024:59.08
  • DOI: 10.33273/2663-4570-2022-92-1-68-83
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LI Medved’s Research Centre of Preventive Toxicology, Food and Chemical Safety, Ministry of Health, Ukraine (State Enterprise), Kyiv, Ukraine


Abstract. The herbicide propisochlor is widely used in agriculture of Ukraine. Its intensive usage is potentially dangerous due to residues in agricultural products, ecosystems, as well as effects on animals’ and humans’ organisms. This fact necessitates the study of its toxicological properties.

Aim. To study the propisochlor carcinogenicity in a long-term experiment on male rats.

Materials and Methods. The experiment was performed on Wistar Han male rats. There was investigated a sample of 95 % technicalgrade propisochlor, administered to animals intragastrically at doses of 20 and 200 mg/kg of body weight for two years. At the 52ndweek-term, a group of animals was selected to study the propisochlor toxicity. Carcinogenicity was assessed at the 104th week.

Results. The toxic effect of propisochlor on the rats’ bodies was revealed in weight loss affected by a 200 mg/kg dose, starting from the 29th week of testing. According to the analysis results of the clinical biochemistry, it was found that propisochlor at a dose of 200 mg/kg at the 52nd week of exposure tended to decrease the activity of ALT and AST. Histologically at the terminal stage at a dose of 200 mg/kg in rats the hepatocellular necrosis with associated focal hemorrhages were detected, which confirmed hepatotoxicity; and in the thyroid gland it was found the increase of nodular follicular-cell hyperplasia cases. Propisochlor did not affect the survival and life expectancy of rats, did not increase the number of animals with tumors, did not affect the multiplicity of newgrowth, did not change the ratio of benign and malignant tumors, and also did not reduce their latency, the dose-effect relationship was not observed.

Conclusions. Propisochlor, 95 % technical-grade generic, did not show the carcinogenic effects in a two-year experiment in male rats. NOAEL was identified according to general toxicological criteria and it was 20 mg/kg.

Key Words: propisochlor, carcinogenesis, toxicology, Wistar Han male rats, long-term experiment.



1. Перелік пестицидів і агрохімікатів, дозволених до використання в Україні. Київ: Довідкове видання; 2020. 896 с.

2. Miklaszewska K, Adamczewski K. Weed control efficacy of propisochlor in wheat, barley, maize, root crops and pea. Journal of Plant Protection Research. 2003;41(4):341–7.

3. ECHA Committee for Risk Assessment, RAC. Opinion proposing harmonised classification and labelling at EU level of Acetochlor (ISO). Adopted 04 December 2014. Доступ:

4. Ki–Hyun Kim, Ehsanul Kabir, Shamin Ara Jahan. Exposure to pesticides and the associated human health effects. Science of the Total Environment. 2017;575:525–35.

5. Kerry D, Nancy M, Alberto P. A survey of EPA. OРР and open literature on selected pesticide chemicals: Mutagenicity and carcinogenicity of selected chloraacetamilids and related compounds. Mutat Res Genetic Toxicol Environ Mutagen. 1999;5:8.

6. Lewis KA, Tzilivakis J, Warner D, Green, A. An international database for pesticide risk assessments and management. Human and Ecological Risk Assessment: an International Journal. 2016;22(4):1050–64.

7. Державні санітарні правила і гігієнічні норми «Гігієнічна класифікація пестицидів за ступенем небезпечності». ДСанПіН–98.

8. European Food Safety Authority; Conclusion on the peer review of the pesticide risk assessment of the active substance propisochlor. EFSA Journal. 2010;8(9):1769.

9. European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes. Strasbourg: Council of Europe; 1986. 51 p.

10. OECD. Guidance document 116 on the Conduct and Design of Chronic Toxicity and carcinogenicity Studies, Supporting Test Guidelines 451, 452 and 453. Series on Testing and Assessment №116. Paris: OECD; 2021. 156 p.

11. GLP. Directive 2004/10/EC, of the European Parliament and of the Council of 11 February 2004. “Statement of GLP compliance №G–042” issued by SNAS 09/03/2015.

12. Turusov VS, Mohr U. Pathology of tumors in laboratory animals. Tumours in the rat. Second Edition. Lyon: IARC Scientific Publications. 1990;1(99):754.

13. Proliferative and Nonproliferative Lesions of the Rat. Toxicological Pathology. 2010;38:5–81.

14. Лапач СН, Губенко АВ, Бабич ПН. Статистические методы в медико–биологических исследованиях с использованием Excel. Киев: Морион; 2001. 408 с.

15. Giknis MLA, Clifford CB. Spontaneous Neoplasms and Survival in Wistar Han Rats: Compilation of Control Group Data. Charles River Laboratories. 2003, 25 р.